Detailed information of TA system TA079556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1014630..1015324
• Replicon: chromosome
• Accession: NZ_CP022689
• Organism: Escherichia coli strain CDC#03-98

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: RM34_RS05940
• Protein ID: WP_001263495.1
• Coordinates: 1014630..1015028 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: RM34_RS05945
• Protein ID: WP_000554757.1
• Coordinates: 1015031..1015324 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1010217..1010297	-	81	NuclAT_12	-	-
-	1010217..1010297	-	81	NuclAT_12	-	-
-	1010217..1010297	-	81	NuclAT_12	-	-
-	1010217..1010297	-	81	NuclAT_12	-	-
RM34_RS05915	1010893..1011351	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
RM34_RS05920	1011612..1013069	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
RM34_RS05925	1013126..1013647	-	522	Protein_965	peptide chain release factor H	-
RM34_RS05930	1013646..1013849	-	204	Protein_966	RNA ligase RtcB family protein	-
RM34_RS05935	1014168..1014620	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
RM34_RS05940	1014630..1015028	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
RM34_RS05945	1015031..1015324	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
RM34_RS05950	1015376..1016431	-	1056	WP_001226188.1	DNA polymerase IV	-
RM34_RS05955	1016502..1017287	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
RM34_RS05960	1017259..1018971	+	1713	Protein_972	flagellar biosynthesis protein FlhA	-
RM34_RS05965	1019116..1019613	-	498	Protein_973	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T79556 WP_001263495.1 NZ_CP022689:c1015028-1014630 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T79556 NZ_CP022689:c1015028-1014630 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT79556 WP_000554757.1 NZ_CP022689:c1015324-1015031 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT79556 NZ_CP022689:c1015324-1015031 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6