Detailed information of TA system TA079538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2022683..2023482
• Replicon: chromosome
• Accession: NZ_CP022686
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: CIW80_RS10080
• Protein ID: WP_000347252.1
• Coordinates: 2023018..2023482 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: CIW80_RS10075
• Protein ID: WP_001296435.1
• Coordinates: 2022683..2023018 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW80_RS10060	2018468..2019238	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
CIW80_RS10065	2019254..2020588	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
CIW80_RS10070	2020963..2022534	+	1572	WP_001273940.1	galactarate dehydratase	-
CIW80_RS10075	2022683..2023018	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CIW80_RS10080	2023018..2023482	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CIW80_RS10085	2023537..2024346	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CIW80_RS10090	2024595..2025875	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CIW80_RS10095	2025898..2026371	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CIW80_RS10100	2026382..2027161	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CIW80_RS10105	2027151..2028029	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CIW80_RS10110	2028047..2028481	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T79538 WP_000347252.1 NZ_CP022686:2023018-2023482 [Escherichia coli Nissle 1917]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T79538 NZ_CP022686:2023018-2023482 [Escherichia coli Nissle 1917]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT79538 WP_001296435.1 NZ_CP022686:2022683-2023018 [Escherichia coli Nissle 1917]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT79538 NZ_CP022686:2022683-2023018 [Escherichia coli Nissle 1917]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5