Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1445144..1445871 Replicon chromosome
Accession NZ_CP022686
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag CIW80_RS07210 Protein ID WP_000547555.1
Coordinates 1445560..1445871 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag CIW80_RS07205 Protein ID WP_000126293.1
Coordinates 1445144..1445563 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIW80_RS07185 1441307..1443559 - 2253 WP_001107659.1 carbamoyltransferase HypF -
CIW80_RS07190 1443712..1444239 - 528 WP_001078777.1 electron transport protein HydN -
CIW80_RS07200 1444624..1445052 + 429 Protein_1321 hypothetical protein -
CIW80_RS07205 1445144..1445563 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
CIW80_RS07210 1445560..1445871 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
CIW80_RS07215 1446034..1446789 - 756 WP_001305268.1 hypothetical protein -
CIW80_RS07220 1446832..1447302 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
CIW80_RS07225 1447295..1447705 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
CIW80_RS07230 1447702..1448469 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
CIW80_RS07235 1448469..1449011 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
CIW80_RS07240 1449021..1450730 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T79535 WP_000547555.1 NZ_CP022686:c1445871-1445560 [Escherichia coli Nissle 1917]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T79535 NZ_CP022686:c1445871-1445560 [Escherichia coli Nissle 1917]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT79535 WP_000126293.1 NZ_CP022686:c1445563-1445144 [Escherichia coli Nissle 1917]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT79535 NZ_CP022686:c1445563-1445144 [Escherichia coli Nissle 1917]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References