Detailed information of TA system TA079500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3766416..3767143
• Replicon: chromosome
• Accession: NZ_CP022672
• Organism: Shigella sonnei strain 866

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CH310_RS19190
• Protein ID: WP_000547563.1
• Coordinates: 3766832..3767143 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CH310_RS19185
• Protein ID: WP_000126296.1
• Coordinates: 3766416..3766835 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH310_RS19165 (3761467)	3761467..3761994	-	528	WP_001078777.1	electron transport protein HydN	-
CH310_RS19170 (3762142)	3762142..3763152	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
CH310_RS19175 (3763412)	3763412..3764869	+	1458	WP_134806831.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CH310_RS19180 (3764878)	3764878..3766302	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
CH310_RS19185 (3766416)	3766416..3766835	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
CH310_RS19190 (3766832)	3766832..3767143	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CH310_RS19195 (3767305)	3767305..3768078	-	774	WP_001026444.1	hypothetical protein	-
CH310_RS19200 (3768103)	3768103..3768573	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CH310_RS19205 (3768566)	3768566..3768976	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CH310_RS19210 (3768973)	3768973..3769740	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
CH310_RS19215 (3769740)	3769740..3770282	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CH310_RS19220 (3770292)	3770292..3771989	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T79500 WP_000547563.1 NZ_CP022672:c3767143-3766832 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T79500 NZ_CP022672:c3767143-3766832 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT79500 WP_000126296.1 NZ_CP022672:c3766835-3766416 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT79500 NZ_CP022672:c3766835-3766416 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22