Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1039095..1039636 Replicon chromosome
Accession NZ_CP022672
Organism Shigella sonnei strain 866

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CH310_RS05030 Protein ID WP_000615976.1
Coordinates 1039095..1039373 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CH310_RS05035 Protein ID WP_000729704.1
Coordinates 1039376..1039636 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH310_RS05015 (1036677) 1036677..1037255 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CH310_RS05020 (1037461) 1037461..1038228 + 768 WP_000333380.1 class II glutamine amidotransferase -
CH310_RS05025 (1038199) 1038199..1038939 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
CH310_RS05030 (1039095) 1039095..1039373 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CH310_RS05035 (1039376) 1039376..1039636 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CH310_RS05040 (1039822) 1039822..1040595 + 774 WP_127148796.1 C40 family peptidase -
CH310_RS05045 (1040884) 1040884..1041192 - 309 Protein_969 IS1-like element IS1A family transposase -
CH310_RS05050 (1041251) 1041251..1042048 - 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
CH310_RS05055 (1042048) 1042048..1043220 - 1173 WP_000952334.1 IS21-like element IS21 family transposase -
CH310_RS05060 (1043234) 1043234..1043715 - 482 Protein_972 IS1 family transposase -
CH310_RS05065 (1043770) 1043770..1043898 + 129 Protein_973 ISAs1 family transposase -
CH310_RS05070 (1043969) 1043969..1044466 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T79487 WP_000615976.1 NZ_CP022672:c1039373-1039095 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T79487 NZ_CP022672:c1039373-1039095 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT79487 WP_000729704.1 NZ_CP022672:c1039636-1039376 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT79487 NZ_CP022672:c1039636-1039376 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References