Detailed information of TA system TA079474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3803506..3804200
• Replicon: chromosome
• Accession: NZ_CP022664
• Organism: Escherichia coli strain FORC 064

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: FORC64_RS20075
• Protein ID: WP_001263489.1
• Coordinates: 3803506..3803904 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FORC64_RS20080
• Protein ID: WP_000554758.1
• Coordinates: 3803907..3804200 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3799094..3799174	-	81	NuclAT_12	-	-
-	3799094..3799174	-	81	NuclAT_12	-	-
-	3799094..3799174	-	81	NuclAT_12	-	-
-	3799094..3799174	-	81	NuclAT_12	-	-
FORC64_RS20045	3799770..3800228	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FORC64_RS20050	3800489..3801946	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
FORC64_RS20055	3802003..3802524	-	522	Protein_3660	peptide chain release factor H	-
FORC64_RS20060	3802520..3802726	-	207	Protein_3661	RtcB family protein	-
FORC64_RS20070	3803044..3803496	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
FORC64_RS20075	3803506..3803904	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FORC64_RS20080	3803907..3804200	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FORC64_RS20085	3804252..3805307	-	1056	WP_001226164.1	DNA polymerase IV	-
FORC64_RS20090	3805378..3806163	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
FORC64_RS20095	3806135..3807847	+	1713	Protein_3667	flagellar biosynthesis protein FlhA	-
FORC64_RS20100	3808071..3808568	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssA / hcp1/tssD1	3803506..3831714	28208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T79474 WP_001263489.1 NZ_CP022664:c3803904-3803506 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T79474 NZ_CP022664:c3803904-3803506 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT79474 WP_000554758.1 NZ_CP022664:c3804200-3803907 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT79474 NZ_CP022664:c3804200-3803907 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3