Detailed information of TA system TA079446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 44381..44983
• Replicon: plasmid pRM11060-1
• Accession: NZ_CP022659
• Organism: Salmonella enterica subsp. enterica strain RM11060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: CGA23_RS25240
• Protein ID: WP_001159630.1
• Coordinates: 44672..44983 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CGA23_RS25235
• Protein ID: WP_000362050.1
• Coordinates: 44381..44671 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGA23_RS25220	41874..42776	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
CGA23_RS25225	42773..43408	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
CGA23_RS25230	43405..44334	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
CGA23_RS25235	44381..44671	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
CGA23_RS25240	44672..44983	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
CGA23_RS25245	45201..46130	+	930	WP_021294279.1	alpha/beta hydrolase	-
CGA23_RS25250	46216..46527	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
CGA23_RS25255	46524..46970	+	447	WP_001259012.1	type II toxin-antitoxin system HigA family antitoxin	-
CGA23_RS25260	46985..47926	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
CGA23_RS25265	47971..48408	-	438	WP_001621365.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CGA23_RS25270	48405..49277	-	873	WP_000921423.1	virulence factor BrkB family protein	-
CGA23_RS25275	49435..49677	-	243	WP_001251454.1	ogr/Delta-like zinc finger family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..70062	70062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T79446 WP_001159630.1 NZ_CP022659:c44983-44672 [Salmonella enterica subsp. enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T79446 NZ_CP022659:c44983-44672 [Salmonella enterica subsp. enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT79446 WP_000362050.1 NZ_CP022659:c44671-44381 [Salmonella enterica subsp. enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT79446 NZ_CP022659:c44671-44381 [Salmonella enterica subsp. enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64