Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1430398..1431317 Replicon chromosome
Accession NZ_CP022577
Organism Mycobacterium tuberculosis strain WC078

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag CHR51_RS07115 Protein ID WP_003900449.1
Coordinates 1430398..1431003 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag CHR51_RS07120 Protein ID WP_003410124.1
Coordinates 1431012..1431317 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CHR51_RS07095 1427886..1428782 + 897 WP_003410136.1 hypothetical protein -
CHR51_RS07100 1428989..1429225 + 237 WP_003410133.1 hypothetical protein -
CHR51_RS07105 1429177..1429854 + 678 WP_003905725.1 hypothetical protein -
CHR51_RS07110 1430014..1430373 + 360 WP_003410131.1 hypothetical protein -
CHR51_RS07115 1430398..1431003 + 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CHR51_RS07120 1431012..1431317 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
CHR51_RS07125 1431402..1431701 + 300 WP_003410120.1 hypothetical protein -
CHR51_RS07130 1431717..1432133 - 417 WP_003410114.1 hypothetical protein -
CHR51_RS07135 1432123..1432842 - 720 WP_003410108.1 DUF433 domain-containing protein -
CHR51_RS07140 1433084..1434124 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
CHR51_RS07145 1434121..1434471 - 351 WP_003918359.1 hypothetical protein -
CHR51_RS07150 1434549..1435810 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
CHR51_RS07155 1435815..1436090 - 276 WP_016330411.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T79272 WP_003900449.1 NZ_CP022577:1430398-1431003 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T79272 NZ_CP022577:1430398-1431003 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT79272 WP_003410124.1 NZ_CP022577:1431012-1431317 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT79272 NZ_CP022577:1431012-1431317 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References