Detailed information of TA system TA079230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 237679..238204
• Replicon: plasmid pSK02
• Accession: NZ_CP022566
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1148

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: CHY08_RS27925
• Protein ID: WP_094088499.1
• Coordinates: 237679..237951 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7V8H8J0
• Locus tag: CHY08_RS27930
• Protein ID: WP_017957817.1
• Coordinates: 237953..238204 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHY08_RS27905	233079..233534	+	456	WP_094088495.1	Rrf2 family transcriptional regulator	-
CHY08_RS27910	233880..235670	+	1791	WP_094088496.1	ABC transporter ATP-binding protein/permease	-
CHY08_RS27915	235746..236225	-	480	WP_094088497.1	class I SAM-dependent methyltransferase	-
CHY08_RS27920	236398..237624	+	1227	WP_094088498.1	DUF3095 domain-containing protein	-
CHY08_RS27925	237679..237951	-	273	WP_094088499.1	Txe/YoeB family addiction module toxin	Toxin
CHY08_RS27930	237953..238204	-	252	WP_017957817.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CHY08_RS27935	238340..239677	-	1338	WP_094088500.1	nucleotide sugar dehydrogenase	-
CHY08_RS27940	240118..241461	+	1344	WP_094088501.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..549819	549819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10787.44 Da
• Isoelectric Point: 9.4530

>T79230 WP_094088499.1 NZ_CP022566:c237951-237679 [Rhizobium leguminosarum bv. viciae]
MKLLWTPNSWEEYEYWQKNDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGFWSRRILGEHRLVYCVTGKGANQQLE
IIQCRFHYEK

Nucleotide

• Length: 273 bp

>T79230 NZ_CP022566:c237951-237679 [Rhizobium leguminosarum bv. viciae]
ATGAAATTGCTATGGACCCCTAATTCCTGGGAAGAGTACGAATACTGGCAGAAAAACGACCAGAAGATGGTCGAGAAAAT
CAACGAACTCCTGAAGGATACAAAAAGGTCACCCTTTAAAGGTCTGGGCAAGCCAGAGCCACTGAAGGGCGATCTATCCG
GATTTTGGTCAAGGCGCATACTCGGAGAGCATCGGCTCGTCTACTGCGTCACTGGCAAGGGTGCCAACCAGCAGCTGGAA
ATCATCCAGTGCCGATTTCACTACGAAAAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9287.55 Da
• Isoelectric Point: 5.6513

>AT79230 WP_017957817.1 NZ_CP022566:c238204-237953 [Rhizobium leguminosarum bv. viciae]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLVAEGEYESMQETLHLLSTPANSARLRESIGQLRKGQVIESD
PTL

Nucleotide

• Length: 252 bp

>AT79230 NZ_CP022566:c238204-237953 [Rhizobium leguminosarum bv. viciae]
ATGGCCCATGTTCGTTTGACGGAGTTCCGGCAAAACATTGCAACCTATTTTGATCAAGTCCTCTCTTCTCGGGCTCCTCT
GCTTGTGACCCGTCAAGGCTCGGAAGCAATGGTCCTGGTTGCCGAAGGCGAGTACGAGAGTATGCAAGAGACGCTACACC
TGCTCTCGACGCCTGCTAATTCTGCCCGGCTACGCGAAAGTATAGGACAGCTTCGAAAGGGGCAGGTGATAGAGAGCGAT
CCAACTCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8H8J0