Detailed information of TA system TA079184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 565028..565755
• Replicon: chromosome
• Accession: NZ_CP022532
• Organism: Enterobacter hormaechei strain MS7884A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0Q486
• Locus tag: MS7884_RS02755
• Protein ID: WP_033486526.1
• Coordinates: 565028..565339 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS7884_RS02760
• Protein ID: WP_033486527.1
• Coordinates: 565336..565755 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS7884_RS02745	560296..561987	-	1692	WP_033486522.1	hypothetical protein	-
MS7884_RS02750	562486..564606	+	2121	WP_033486523.1	hypothetical protein	-
MS7884_RS02755	565028..565339	+	312	WP_033486526.1	type II toxin-antitoxin system HigB family toxin	Toxin
MS7884_RS02760	565336..565755	+	420	WP_033486527.1	helix-turn-helix domain-containing protein	Antitoxin
MS7884_RS02765	565808..566698	-	891	WP_033486528.1	oxidoreductase	-
MS7884_RS02770	566804..567925	+	1122	WP_032670426.1	glutamate--cysteine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12506.30 Da
• Isoelectric Point: 9.9435

>T79184 WP_033486526.1 NZ_CP022532:565028-565339 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T79184 NZ_CP022532:565028-565339 [Enterobacter hormaechei]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCTAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTGATGTTTTTTGCTGATTTTGAGCGTGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCACGGACTACTACCGGAGAACGAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15341.43 Da
• Isoelectric Point: 4.7132

>AT79184 WP_033486527.1 NZ_CP022532:565336-565755 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPLLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT79184 NZ_CP022532:565336-565755 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTACTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTAGAGTATCTTATCGAGCACGACCCCGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTATGAAAACGCGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGCATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTTAACGGACAGCGCACGTTAACGCTAGATCATATGCGAGCGCTGGCGAAGCGTTTCGGTTTGCCGGTGA
GCGCATTTGTGGGGAACTGA

Structures

Toxin

• AlphaFold DB: A0A2J0Q486