Detailed information of TA system TA079113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4492934..4493529
• Replicon: chromosome
• Accession: NZ_CP022478
• Organism: Pseudomonas aeruginosa strain LW

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CEK59_RS21400
• Protein ID: WP_003113526.1
• Coordinates: 4493251..4493529 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CEK59_RS21395
• Protein ID: WP_031638452.1
• Coordinates: 4492934..4493239 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEK59_RS21360	4488074..4488922	+	849	WP_009316211.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CEK59_RS21370	4489089..4490030	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CEK59_RS21375	4490147..4490761	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
CEK59_RS21380	4490803..4491387	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CEK59_RS21385	4491428..4492528	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CEK59_RS21395	4492934..4493239	-	306	WP_031638452.1	HigA family addiction module antidote protein	Antitoxin
CEK59_RS21400	4493251..4493529	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEK59_RS21410	4493858..4496086	+	2229	WP_058160584.1	TonB-dependent receptor	-
CEK59_RS21415	4496157..4496804	-	648	WP_003095021.1	carbonate dehydratase	-
CEK59_RS21420	4496866..4498104	-	1239	WP_034036794.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T79113 WP_003113526.1 NZ_CP022478:c4493529-4493251 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T79113 NZ_CP022478:c4493529-4493251 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11156.70 Da
• Isoelectric Point: 4.7860

>AT79113 WP_031638452.1 NZ_CP022478:c4493239-4492934 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELGISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT79113 NZ_CP022478:c4493239-4492934 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGGTATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACACGAGATTGAGCCGCTGCTTGCCCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3