Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2325605..2326146 Replicon chromosome
Accession NZ_CP022457
Organism Shigella sonnei strain 2015C-3566

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag B9128_RS12565 Protein ID WP_000615976.1
Coordinates 2325605..2325883 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag B9128_RS12570 Protein ID WP_000729704.1
Coordinates 2325886..2326146 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9128_RS12550 (2323187) 2323187..2323765 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
B9128_RS12555 (2323971) 2323971..2324738 + 768 WP_000333380.1 class II glutamine amidotransferase -
B9128_RS12560 (2324709) 2324709..2325449 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
B9128_RS12565 (2325605) 2325605..2325883 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B9128_RS12570 (2325886) 2325886..2326146 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B9128_RS12575 (2326332) 2326332..2327105 + 774 Protein_2243 C40 family peptidase -
B9128_RS27410 (2328146) 2328146..2328274 + 129 Protein_2245 ISAs1 family transposase -
B9128_RS12590 (2328345) 2328345..2328842 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
B9128_RS12595 (2328965) 2328965..2330677 - 1713 Protein_2247 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2315455..2328842 13387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T79084 WP_000615976.1 NZ_CP022457:c2325883-2325605 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T79084 NZ_CP022457:c2325883-2325605 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT79084 WP_000729704.1 NZ_CP022457:c2326146-2325886 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT79084 NZ_CP022457:c2326146-2325886 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References