Detailed information of TA system TA079079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 207317..208044
• Replicon: chromosome
• Accession: NZ_CP022457
• Organism: Shigella sonnei strain 2015C-3566

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: B9128_RS01260
• Protein ID: WP_000547563.1
• Coordinates: 207733..208044 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9128_RS01255
• Protein ID: WP_000126296.1
• Coordinates: 207317..207736 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9128_RS01235 (202368)	202368..202895	-	528	WP_001078766.1	electron transport protein HydN	-
B9128_RS01240 (203043)	203043..204053	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
B9128_RS01245 (204313)	204313..205770	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
B9128_RS01250 (205779)	205779..207203	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
B9128_RS01255 (207317)	207317..207736	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
B9128_RS01260 (207733)	207733..208044	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
B9128_RS01265 (208206)	208206..208979	-	774	WP_001026459.1	hypothetical protein	-
B9128_RS01270 (209004)	209004..209474	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
B9128_RS01275 (209467)	209467..209877	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
B9128_RS01280 (209874)	209874..210641	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
B9128_RS01285 (210641)	210641..211183	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
B9128_RS01290 (211193)	211193..212890	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T79079 WP_000547563.1 NZ_CP022457:c208044-207733 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T79079 NZ_CP022457:c208044-207733 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT79079 WP_000126296.1 NZ_CP022457:c207736-207317 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT79079 NZ_CP022457:c207736-207317 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22