Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4122771..4123312 Replicon chromosome
Accession NZ_CP022455
Organism Shigella sonnei strain 2015C-3794

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag B9129_RS22690 Protein ID WP_000615976.1
Coordinates 4122771..4123049 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag B9129_RS22695 Protein ID WP_000729704.1
Coordinates 4123052..4123312 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9129_RS22675 (4120353) 4120353..4120931 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
B9129_RS22680 (4121137) 4121137..4121904 + 768 WP_000333380.1 class II glutamine amidotransferase -
B9129_RS22685 (4121875) 4121875..4122615 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
B9129_RS22690 (4122771) 4122771..4123049 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
B9129_RS22695 (4123052) 4123052..4123312 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
B9129_RS22700 (4123498) 4123498..4124271 + 774 WP_127148796.1 C40 family peptidase -
B9129_RS27330 (4125312) 4125312..4125440 + 129 Protein_4074 ISAs1 family transposase -
B9129_RS22715 (4125511) 4125511..4126008 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
B9129_RS22720 (4126131) 4126131..4127843 - 1713 Protein_4076 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4112621..4126008 13387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T79072 WP_000615976.1 NZ_CP022455:c4123049-4122771 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T79072 NZ_CP022455:c4123049-4122771 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT79072 WP_000729704.1 NZ_CP022455:c4123312-4123052 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT79072 NZ_CP022455:c4123312-4123052 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References