Detailed information of TA system TA079067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2006723..2007450
• Replicon: chromosome
• Accession: NZ_CP022455
• Organism: Shigella sonnei strain 2015C-3794

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: B9129_RS11425
• Protein ID: WP_000547563.1
• Coordinates: 2007139..2007450 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B9129_RS11420
• Protein ID: WP_000126296.1
• Coordinates: 2006723..2007142 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9129_RS11400 (2001774)	2001774..2002301	-	528	WP_001078766.1	electron transport protein HydN	-
B9129_RS11405 (2002449)	2002449..2003459	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
B9129_RS11410 (2003719)	2003719..2005176	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
B9129_RS11415 (2005185)	2005185..2006609	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
B9129_RS11420 (2006723)	2006723..2007142	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
B9129_RS11425 (2007139)	2007139..2007450	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
B9129_RS11430 (2007612)	2007612..2008385	-	774	WP_001026444.1	hypothetical protein	-
B9129_RS11435 (2008410)	2008410..2008880	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
B9129_RS11440 (2008873)	2008873..2009283	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
B9129_RS11445 (2009280)	2009280..2010047	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
B9129_RS11450 (2010047)	2010047..2010589	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
B9129_RS11455 (2010599)	2010599..2012296	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T79067 WP_000547563.1 NZ_CP022455:c2007450-2007139 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T79067 NZ_CP022455:c2007450-2007139 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT79067 WP_000126296.1 NZ_CP022455:c2007142-2006723 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT79067 NZ_CP022455:c2007142-2006723 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22