Detailed information of TA system TA078988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4969952..4970679
• Replicon: chromosome
• Accession: NZ_CP022407
• Organism: Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: CGC46_RS26385
• Protein ID: WP_000547564.1
• Coordinates: 4969952..4970263 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CGC46_RS26390
• Protein ID: WP_000126294.1
• Coordinates: 4970260..4970679 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC46_RS26355	4965094..4966803	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
CGC46_RS26360	4966813..4967355	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
CGC46_RS26365	4967355..4968122	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
CGC46_RS26370	4968119..4968529	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CGC46_RS26375	4968522..4968992	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CGC46_RS26380	4969017..4969778	+	762	WP_001026445.1	hypothetical protein	-
CGC46_RS26385	4969952..4970263	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
CGC46_RS26390	4970260..4970679	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CGC46_RS26395	4970794..4972218	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
CGC46_RS26400	4972227..4973603	-	1377	WP_001107877.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CGC46_RS26405	4973863..4974873	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
CGC46_RS26410	4975022..4975549	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T78988 WP_000547564.1 NZ_CP022407:4969952-4970263 [Escherichia coli O121:H19]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T78988 NZ_CP022407:4969952-4970263 [Escherichia coli O121:H19]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT78988 WP_000126294.1 NZ_CP022407:4970260-4970679 [Escherichia coli O121:H19]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT78988 NZ_CP022407:4970260-4970679 [Escherichia coli O121:H19]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5