Detailed information of TA system TA078979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3589097..3589699
• Replicon: chromosome
• Accession: NZ_CP022407
• Organism: Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CGC46_RS19150
• Protein ID: WP_000897305.1
• Coordinates: 3589388..3589699 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CGC46_RS19145
• Protein ID: WP_000356397.1
• Coordinates: 3589097..3589387 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC46_RS19115	3585042..3585944	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CGC46_RS19120	3585941..3586576	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CGC46_RS19125	3586573..3587502	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CGC46_RS19130	3587832..3588074	-	243	WP_001086388.1	hypothetical protein	-
CGC46_RS19135	3588293..3588511	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CGC46_RS19145	3589097..3589387	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CGC46_RS19150	3589388..3589699	-	312	WP_000897305.1	hypothetical protein	Toxin
CGC46_RS19155	3589928..3590835	+	908	Protein_3518	alpha/beta hydrolase	-
CGC46_RS19165	3591003..3591917	-	915	WP_072097029.1	transposase	-
CGC46_RS19170	3591930..3592868	-	939	Protein_3520	hypothetical protein	-
CGC46_RS19175	3593232..3594173	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CGC46_RS19180	3594218..3594655	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T78979 WP_000897305.1 NZ_CP022407:c3589699-3589388 [Escherichia coli O121:H19]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T78979 NZ_CP022407:c3589699-3589388 [Escherichia coli O121:H19]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT78979 WP_000356397.1 NZ_CP022407:c3589387-3589097 [Escherichia coli O121:H19]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT78979 NZ_CP022407:c3589387-3589097 [Escherichia coli O121:H19]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7