Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2635214..2635755 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CGC46_RS14280 Protein ID WP_000615976.1
Coordinates 2635477..2635755 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A4T9JVH9
Locus tag CGC46_RS14275 Protein ID WP_000729706.1
Coordinates 2635214..2635474 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS14250 2630997..2631782 - 786 WP_000207558.1 putative lateral flagellar export/assembly protein LafU -
CGC46_RS14255 2631754..2633466 + 1713 Protein_2634 flagellar biosynthesis protein FlhA -
CGC46_RS14260 2633571..2633849 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CGC46_RS14265 2633842..2634198 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CGC46_RS14270 2634255..2635028 - 774 WP_000543899.1 C40 family peptidase -
CGC46_RS14275 2635214..2635474 + 261 WP_000729706.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CGC46_RS14280 2635477..2635755 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CGC46_RS14285 2635911..2636651 + 741 WP_001225679.1 murein L,D-transpeptidase -
CGC46_RS14290 2636622..2637389 - 768 WP_000333380.1 class II glutamine amidotransferase -
CGC46_RS14295 2637595..2638173 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T78976 WP_000615976.1 NZ_CP022407:2635477-2635755 [Escherichia coli O121:H19]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T78976 NZ_CP022407:2635477-2635755 [Escherichia coli O121:H19]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT78976 WP_000729706.1 NZ_CP022407:2635214-2635474 [Escherichia coli O121:H19]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADNL
FDKLGI

Download         Length: 261 bp

>AT78976 NZ_CP022407:2635214-2635474 [Escherichia coli O121:H19]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATAATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A4T9JVH9

References