Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3805245..3805747 Replicon chromosome
Accession NZ_CP022393
Organism Escherichia coli strain E62

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CEJ55_RS19470 Protein ID WP_000767829.1
Coordinates 3805245..3805499 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CEJ55_RS19475 Protein ID WP_001259255.1
Coordinates 3805496..3805747 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEJ55_RS19445 3800260..3800487 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CEJ55_RS19450 3800501..3801559 - 1059 WP_000492346.1 transport protein YeeE -
CEJ55_RS19455 3801738..3803096 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CEJ55_RS25385 3803086..3803148 - 63 WP_010723108.1 membrane protein YoeI -
CEJ55_RS19460 3803363..3804292 - 930 WP_000803351.1 LysR family transcriptional regulator -
CEJ55_RS19465 3804338..3805162 - 825 WP_021562277.1 SDR family oxidoreductase -
CEJ55_RS19470 3805245..3805499 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CEJ55_RS19475 3805496..3805747 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CEJ55_RS19480 3806030..3806080 + 51 WP_001364200.1 his operon leader peptide -
CEJ55_RS19490 3806226..3807125 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CEJ55_RS19495 3807131..3808435 + 1305 WP_001570037.1 histidinol dehydrogenase -
CEJ55_RS19500 3808432..3809502 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
CEJ55_RS19505 3809502..3810569 + 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T78957 WP_000767829.1 NZ_CP022393:c3805499-3805245 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T78957 NZ_CP022393:c3805499-3805245 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT78957 WP_001259255.1 NZ_CP022393:c3805747-3805496 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT78957 NZ_CP022393:c3805747-3805496 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References