Detailed information of TA system TA078936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 318669..319271
• Replicon: chromosome
• Accession: NZ_CP022393
• Organism: Escherichia coli strain E62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CEJ55_RS01575
• Protein ID: WP_000897305.1
• Coordinates: 318960..319271 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CEJ55_RS01570
• Protein ID: WP_000356397.1
• Coordinates: 318669..318959 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEJ55_RS01540	314613..315515	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CEJ55_RS01545	315512..316147	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CEJ55_RS01550	316144..317073	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CEJ55_RS01555	317403..317645	-	243	WP_001087409.1	hypothetical protein	-
CEJ55_RS01560	317865..318083	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CEJ55_RS01570	318669..318959	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CEJ55_RS01575	318960..319271	-	312	WP_000897305.1	hypothetical protein	Toxin
CEJ55_RS01580	319500..320408	+	909	WP_001162704.1	alpha/beta hydrolase	-
CEJ55_RS01585	320472..321413	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CEJ55_RS01590	321458..321895	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CEJ55_RS01595	321892..322764	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CEJ55_RS01600	322758..323357	-	600	WP_001295269.1	glucose-1-phosphatase	-
CEJ55_RS01605	323456..324241	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T78936 WP_000897305.1 NZ_CP022393:c319271-318960 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T78936 NZ_CP022393:c319271-318960 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT78936 WP_000356397.1 NZ_CP022393:c318959-318669 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT78936 NZ_CP022393:c318959-318669 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7