Detailed information of TA system TA078922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 155233..155890
• Replicon: plasmid pSHE-CTX-M
• Accession: NZ_CP022359
• Organism: Shewanella bicestrii strain JAB-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CF168_RS21905
• Protein ID: WP_000270043.1
• Coordinates: 155540..155890 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CF168_RS21900
• Protein ID: WP_000124640.1
• Coordinates: 155233..155535 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF168_RS21850	150858..151286	+	429	WP_000591074.1	hypothetical protein	-
CF168_RS21855	151343..151702	+	360	WP_000422768.1	hypothetical protein	-
CF168_RS21860	151702..152148	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
CF168_RS21865	152145..152663	+	519	WP_000210756.1	nitrite reductase	-
CF168_RS21870	152663..152893	+	231	WP_000972663.1	hypothetical protein	-
CF168_RS21875	152880..153737	+	858	WP_001167032.1	hypothetical protein	-
CF168_RS21885	153968..154495	+	528	WP_001236377.1	thermonuclease family protein	-
CF168_RS21890	154553..154825	+	273	WP_001043047.1	HU family DNA-binding protein	-
CF168_RS21895	154913..155206	+	294	WP_001239997.1	chromosome segregation protein ParM	-
CF168_RS21900	155233..155535	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CF168_RS21905	155540..155890	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CF168_RS21910	156053..156601	+	549	WP_001061574.1	transcriptional regulator	-
CF168_RS21920	156942..157136	+	195	WP_000343597.1	hypothetical protein	-
CF168_RS21925	157147..157518	+	372	WP_000516916.1	hypothetical protein	-
CF168_RS21930	157511..157981	+	471	WP_001281821.1	hypothetical protein	-
CF168_RS21935	157996..158331	-	336	WP_000683476.1	hypothetical protein	-
CF168_RS21940	158428..158916	+	489	WP_167373725.1	DUF1643 domain-containing protein	-
CF168_RS21945	158919..159416	+	498	WP_000062185.1	hypothetical protein	-
CF168_RS21950	159631..160335	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / tet(A) / blaSHV-12 / aph(6)-Id / aph(3'')-Ib / sul2	-	1..193338	193338

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T78922 WP_000270043.1 NZ_CP022359:c155890-155540 [Shewanella bicestrii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T78922 NZ_CP022359:c155890-155540 [Shewanella bicestrii]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT78922 WP_000124640.1 NZ_CP022359:c155535-155233 [Shewanella bicestrii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT78922 NZ_CP022359:c155535-155233 [Shewanella bicestrii]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9