Detailed information of TA system TA078867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 79074..79725
• Replicon: plasmid pIC001A
• Accession: NZ_CP022299
• Organism: Acinetobacter johnsonii strain IC001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CFH90_RS18165
• Protein ID: WP_126039492.1
• Coordinates: 79074..79430 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CFH90_RS18170
• Protein ID: WP_001140620.1
• Coordinates: 79423..79725 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFH90_RS18150	74570..75100	+	531	WP_126039489.1	plasmid replication DNA-binding protein	-
CFH90_RS18155	75315..76769	-	1455	WP_062032131.1	SulP family inorganic anion transporter	-
CFH90_RS18160	77175..78509	+	1335	WP_126034967.1	ISNCY family transposase	-
CFH90_RS18165	79074..79430	+	357	WP_126039492.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFH90_RS18170	79423..79725	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
CFH90_RS18175	80278..81410	-	1133	Protein_82	IS3 family transposase	-
CFH90_RS18180	81761..81976	+	216	WP_004637138.1	cold-shock protein	-
CFH90_RS18185	82075..82302	+	228	WP_126039499.1	hypothetical protein	-
CFH90_RS18190	82372..82569	+	198	WP_004637135.1	hypothetical protein	-
CFH90_RS18195	82891..84009	+	1119	WP_126039520.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..94476	94476
	-	inside	IScluster/Tn	-	-	77187..81410	4223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T78867 WP_126039492.1 NZ_CP022299:79074-79430 [Acinetobacter johnsonii]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHITTLGDKSNG

Nucleotide

• Length: 357 bp

>T78867 NZ_CP022299:79074-79430 [Acinetobacter johnsonii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAATCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTCGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGCCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGCGTTCAACACCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATATCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT78867 WP_001140620.1 NZ_CP022299:79423-79725 [Acinetobacter johnsonii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT78867 NZ_CP022299:79423-79725 [Acinetobacter johnsonii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA