Detailed information of TA system TA078820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 139044..139629
• Replicon: plasmid pAba7804b
• Accession: NZ_CP022285
• Organism: Acinetobacter baumannii strain 7804

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7S7BY50
• Locus tag: Aba7804_RS20940
• Protein ID: WP_000897311.1
• Coordinates: 139044..139364 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Aba7804_RS20945
• Protein ID: WP_000369784.1
• Coordinates: 139357..139629 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Aba7804_RS20910	134851..135180	+	330	WP_000506885.1	DUF4236 domain-containing protein	-
Aba7804_RS20920	135452..136684	-	1233	WP_001255535.1	ATP-binding protein	-
Aba7804_RS20930	136942..137379	-	438	Protein_126	IS3 family transposase	-
Aba7804_RS20935	137433..138365	+	933	WP_000743272.1	IS5-like element IS17 family transposase	-
Aba7804_RS20940	139044..139364	+	321	WP_000897311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Aba7804_RS20945	139357..139629	+	273	WP_000369784.1	helix-turn-helix domain-containing protein	Antitoxin
Aba7804_RS20950	139662..140462	+	801	WP_000604996.1	DUF2971 domain-containing protein	-
Aba7804_RS20955	140553..140854	+	302	Protein_131	transposase	-
Aba7804_RS20960	140970..141182	+	213	WP_001123161.1	hypothetical protein	-
Aba7804_RS20965	141508..142364	-	857	Protein_133	LysR family transcriptional regulator	-
Aba7804_RS20970	142361..143368	-	1008	WP_005071472.1	2-hydroxyacid dehydrogenase	-
Aba7804_RS20975	143410..144207	-	798	WP_004757464.1	phosphonate ABC transporter, permease protein PhnE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / tet(B) / aph(6)-Id / aph(3'')-Ib	-	1..170420	170420
	-	inside	IScluster/Tn	-	-	136942..140888	3946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-84)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12214.03 Da
• Isoelectric Point: 7.9500

>T78820 WP_000897311.1 NZ_CP022285:139044-139364 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFNG

Nucleotide

• Length: 321 bp

>T78820 NZ_CP022285:139044-139364 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10145.57 Da
• Isoelectric Point: 7.0146

>AT78820 WP_000369784.1 NZ_CP022285:139357-139629 [Acinetobacter baumannii]
MDNNLFDDLVTSIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDTNPTLI

Nucleotide

• Length: 273 bp

>AT78820 NZ_CP022285:139357-139629 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: A0A7S7BY50