Detailed information of TA system TA078809

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32898..33151
• Replicon: plasmid pSTEC299_1
• Accession: NZ_CP022280
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CFI09_RS25890
• Protein ID: WP_001312851.1
• Coordinates: 33002..33151 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	32898..32954 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS25850	28828..29574	+	747	WP_101973910.1	conjugal transfer pilus acetylase TraX	-
CFI09_RS25855	29629..30189	+	561	WP_001567328.1	fertility inhibition protein FinO	-
CFI09_RS25860	30325..30537	+	213	WP_001299730.1	ANR family transcriptional regulator	-
CFI09_RS25865	30783..31244	+	462	WP_101973909.1	thermonuclease family protein	-
CFI09_RS25870	31290..31499	+	210	WP_101973908.1	hemolysin expression modulator Hha	-
CFI09_RS25875	31537..32127	+	591	WP_101973907.1	DUF2726 domain-containing protein	-
CFI09_RS25880	32282..32746	+	465	WP_101973911.1	hypothetical protein	-
-	32898..32954	-	57	NuclAT_0	-	Antitoxin
-	32898..32954	-	57	NuclAT_0	-	Antitoxin
-	32898..32954	-	57	NuclAT_0	-	Antitoxin
-	32898..32954	-	57	NuclAT_0	-	Antitoxin
CFI09_RS25890	33002..33151	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CFI09_RS25895	33435..33689	+	255	WP_000083830.1	replication regulatory protein RepA	-
CFI09_RS25905	33926..34000	+	75	WP_032144212.1	RepA leader peptide Tap	-
CFI09_RS25910	33993..35015	+	1023	WP_101973912.1	replication initiation protein	-
CFI09_RS25920	35941..36225	+	285	WP_101973913.1	DUF1778 domain-containing protein	-
CFI09_RS25925	36213..36698	+	486	WP_101973914.1	GNAT family N-acetyltransferase	-
CFI09_RS27440	36682..37174	-	493	Protein_42	hypothetical protein	-
CFI09_RS27685	37202..37448	+	247	Protein_43	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	paa / afaC-VII / exeG / stcE	1..191691	191691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T78809 WP_001312851.1 NZ_CP022280:33002-33151 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T78809 NZ_CP022280:33002-33151 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT78809 NZ_CP022280:c32954-32898 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin