Detailed information of TA system TA078807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4692059..4692661
• Replicon: chromosome
• Accession: NZ_CP022279
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CFI09_RS24605
• Protein ID: WP_000897302.1
• Coordinates: 4692350..4692661 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CFI09_RS24600
• Protein ID: WP_000356397.1
• Coordinates: 4692059..4692349 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS24565	4688132..4689034	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CFI09_RS24570	4689031..4689666	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CFI09_RS24575	4689663..4690592	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CFI09_RS24580	4690808..4691026	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
CFI09_RS24590	4691422..4691700	-	279	WP_001296612.1	hypothetical protein	-
CFI09_RS27835	4691762..4691974	-	213	WP_000197774.1	hypothetical protein	-
CFI09_RS24600	4692059..4692349	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CFI09_RS24605	4692350..4692661	-	312	WP_000897302.1	hypothetical protein	Toxin
CFI09_RS24610	4692890..4693798	+	909	WP_001331553.1	alpha/beta hydrolase	-
CFI09_RS24615	4693862..4694803	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CFI09_RS24620	4694848..4695285	-	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CFI09_RS24625	4695282..4696154	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CFI09_RS24630	4696148..4696747	-	600	WP_001314324.1	glucose-1-phosphatase	-
CFI09_RS24635	4696846..4697631	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T78807 WP_000897302.1 NZ_CP022279:c4692661-4692350 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T78807 NZ_CP022279:c4692661-4692350 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT78807 WP_000356397.1 NZ_CP022279:c4692349-4692059 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT78807 NZ_CP022279:c4692349-4692059 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416