Detailed information of TA system TA078802

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4177471..4177883
• Replicon: chromosome
• Accession: NZ_CP022279
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A5E9Z711
• Locus tag: CFI09_RS22095
• Protein ID: WP_000132600.1
• Coordinates: 4177542..4177883 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4177471..4177547 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS22085	4174098..4175567	+	1470	WP_016237704.1	type I restriction-modification system subunit M	-
CFI09_RS22090	4175567..4177321	+	1755	WP_101973827.1	restriction endonuclease subunit S	-
-	4177471..4177547	-	77	NuclAT_5	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_5	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_5	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_5	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_6	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_6	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_6	-	Antitoxin
-	4177471..4177547	-	77	NuclAT_6	-	Antitoxin
CFI09_RS22095	4177542..4177883	+	342	WP_000132600.1	endoribonuclease SymE	Toxin
CFI09_RS22100	4177930..4180023	-	2094	WP_000648236.1	DUF262 domain-containing protein	-
CFI09_RS22105	4180121..4180201	-	81	WP_020233658.1	hypothetical protein	-
CFI09_RS22110	4180429..4181349	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
CFI09_RS22115	4181534..4182814	+	1281	WP_001535800.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4161302..4180086	18784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12251.10 Da
• Isoelectric Point: 9.0999

>T78802 WP_000132600.1 NZ_CP022279:4177542-4177883 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLAAAGFATGTAIDVKVMEGCIVLTAQPTAAEESE
LMQSLRQVCKLSARKQKQVQEFIGVITGKKKVA

Nucleotide

• Length: 342 bp

>T78802 NZ_CP022279:4177542-4177883 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGCAGCCGCCGGTTTTGCCA
CCGGCACGGCGATAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCCACCGCCGCCGAAGAGAGCGAG
CTGATGCAGTCACTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAATTTATTGGGGTGATTAC
AGGTAAAAAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT78802 NZ_CP022279:c4177547-4177471 [Escherichia coli]
AGTCATAACTGCTATTCTCCTGGAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A5E9Z711

Antitoxin