Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3759780..3760474 Replicon chromosome
Accession NZ_CP022279
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag CFI09_RS19950 Protein ID WP_001263501.1
Coordinates 3759780..3760178 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CFI09_RS19955 Protein ID WP_000554755.1
Coordinates 3760181..3760474 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS19925 3755145..3755603 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
CFI09_RS19930 3755864..3757321 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
CFI09_RS19935 3757378..3757992 - 615 WP_000602123.1 peptide chain release factor H -
CFI09_RS19940 3757989..3759128 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
CFI09_RS19945 3759318..3759770 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
CFI09_RS19950 3759780..3760178 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CFI09_RS19955 3760181..3760474 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CFI09_RS19960 3760526..3761581 - 1056 WP_001226167.1 DNA polymerase IV -
CFI09_RS19965 3761652..3762440 - 789 WP_000258901.1 putative lateral flagellar export/assembly protein LafU -
CFI09_RS19970 3762409..3764121 + 1713 Protein_3667 flagellar biosynthesis protein FlhA -
CFI09_RS27405 3764200..3764358 + 159 WP_157848283.1 hypothetical protein -
CFI09_RS27645 3764540..3765037 - 498 Protein_3669 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T78798 WP_001263501.1 NZ_CP022279:c3760178-3759780 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T78798 NZ_CP022279:c3760178-3759780 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT78798 WP_000554755.1 NZ_CP022279:c3760474-3760181 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT78798 NZ_CP022279:c3760474-3760181 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References