Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2509964..2510526 Replicon chromosome
Accession NZ_CP022279
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name higB Uniprot ID A0A786YE86
Locus tag CFI09_RS13185 Protein ID WP_000605674.1
Coordinates 2509964..2510242 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CFI09_RS13190 Protein ID WP_000781370.1
Coordinates 2510242..2510526 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS13160 2505550..2505981 - 432 WP_000152305.1 peroxiredoxin OsmC -
CFI09_RS13165 2506326..2506541 + 216 WP_000495766.1 biofilm-dependent modulation protein -
CFI09_RS13170 2506643..2506780 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CFI09_RS13175 2506937..2508634 + 1698 WP_033552793.1 malate dehydrogenase -
CFI09_RS13180 2508768..2509778 + 1011 WP_033552795.1 alcohol dehydrogenase AdhP -
CFI09_RS13185 2509964..2510242 + 279 WP_000605674.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFI09_RS13190 2510242..2510526 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
CFI09_RS13195 2510755..2511408 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
CFI09_RS13200 2511401..2512285 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
CFI09_RS13205 2512298..2515345 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10609.23 Da        Isoelectric Point: 6.8588

>T78795 WP_000605674.1 NZ_CP022279:2509964-2510242 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDEIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T78795 NZ_CP022279:2509964-2510242 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGAAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTATGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT78795 WP_000781370.1 NZ_CP022279:2510242-2510526 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT78795 NZ_CP022279:2510242-2510526 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A786YE86

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References