Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1763302..1763804 Replicon chromosome
Accession NZ_CP022279
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CFI09_RS08785 Protein ID WP_000767829.1
Coordinates 1763550..1763804 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CFI09_RS08780 Protein ID WP_001259255.1
Coordinates 1763302..1763553 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS08745 1758480..1759547 - 1068 WP_000080081.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CFI09_RS08750 1759547..1760617 - 1071 WP_001099216.1 histidinol-phosphate transaminase -
CFI09_RS08755 1760614..1761918 - 1305 WP_001346884.1 histidinol dehydrogenase -
CFI09_RS08760 1761924..1762823 - 900 WP_000131779.1 ATP phosphoribosyltransferase -
CFI09_RS08770 1762969..1763019 - 51 WP_001364200.1 his operon leader peptide -
CFI09_RS08780 1763302..1763553 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CFI09_RS08785 1763550..1763804 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CFI09_RS08790 1763887..1764711 + 825 WP_000754740.1 SDR family oxidoreductase -
CFI09_RS08795 1764757..1765686 + 930 WP_000803366.1 LysR family transcriptional regulator -
CFI09_RS08800 1765901..1765963 + 63 WP_010723108.1 membrane protein YoeI -
CFI09_RS08805 1765953..1767311 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T78784 WP_000767829.1 NZ_CP022279:1763550-1763804 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T78784 NZ_CP022279:1763550-1763804 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT78784 WP_001259255.1 NZ_CP022279:1763302-1763553 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT78784 NZ_CP022279:1763302-1763553 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References