Detailed information of TA system TA078783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1076631..1077358
• Replicon: chromosome
• Accession: NZ_CP022279
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CFI09_RS05470
• Protein ID: WP_000547563.1
• Coordinates: 1076631..1076942 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CFI09_RS05475
• Protein ID: WP_000126304.1
• Coordinates: 1076939..1077358 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS05440	1071772..1073481	+	1710	WP_001288136.1	formate hydrogenlyase subunit HycE	-
CFI09_RS05445	1073491..1074033	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
CFI09_RS05450	1074033..1074800	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CFI09_RS05455	1074797..1075207	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CFI09_RS05460	1075200..1075670	+	471	WP_000132956.1	hydrogenase maturation peptidase HycI	-
CFI09_RS05465	1075713..1076468	+	756	WP_001553332.1	hypothetical protein	-
CFI09_RS05470	1076631..1076942	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CFI09_RS05475	1076939..1077358	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
CFI09_RS05480	1077450..1077878	-	429	WP_000536064.1	hypothetical protein	-
CFI09_RS05490	1078263..1078790	+	528	WP_001078777.1	electron transport protein HydN	-
CFI09_RS05495	1078943..1081195	+	2253	WP_012601682.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T78783 WP_000547563.1 NZ_CP022279:1076631-1076942 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T78783 NZ_CP022279:1076631-1076942 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT78783 WP_000126304.1 NZ_CP022279:1076939-1077358 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT78783 NZ_CP022279:1076939-1077358 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22