Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 4725352..4725857 Replicon chromosome
Accession NZ_CP022273
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name yoeB Uniprot ID A0A5H6P158
Locus tag CFA70_RS24085 Protein ID WP_023218457.1
Coordinates 4725352..4725612 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A5H6P2W8
Locus tag CFA70_RS24090 Protein ID WP_052932205.1
Coordinates 4725612..4725857 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS24065 4720882..4721475 - 594 WP_003845054.1 Slp family lipoprotein -
CFA70_RS24070 4721599..4721853 - 255 WP_003845055.1 DUF1471 domain-containing protein -
CFA70_RS24075 4722373..4724271 + 1899 WP_088903227.1 methyl-accepting chemotaxis protein -
CFA70_RS24080 4724446..4725075 + 630 WP_003028217.1 DNA-binding protein -
CFA70_RS24085 4725352..4725612 - 261 WP_023218457.1 Txe/YoeB family addiction module toxin Toxin
CFA70_RS24090 4725612..4725857 - 246 WP_052932205.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
CFA70_RS24095 4726340..4726602 + 263 Protein_4508 ogr/Delta-like zinc finger family protein -
CFA70_RS24100 4726795..4727100 - 306 WP_087878867.1 hypothetical protein -
CFA70_RS24105 4727623..4727910 + 288 Protein_4510 hypothetical protein -
CFA70_RS24110 4729361..4730212 + 852 WP_088903228.1 acyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - acrB 4701958..4749044 47086
- flank IS/Tn - - 4730278..4731225 947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-83)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10202.88 Da        Isoelectric Point: 10.2570

>T78761 WP_023218457.1 NZ_CP022273:c4725612-4725352 [Citrobacter freundii]
MKVRISPQAKSDLQYWAETDPKTHNKVMELIKSAKESPFKGIGKPEALKHRKPLWSRRITKEHRLVYYVEQGDLYIVACR
YHYTTI

Download         Length: 261 bp

>T78761 NZ_CP022273:c4725612-4725352 [Citrobacter freundii]
ATGAAAGTCAGGATATCCCCGCAGGCAAAAAGCGATCTTCAATACTGGGCCGAGACTGACCCAAAGACGCATAACAAAGT
AATGGAGTTAATCAAATCGGCTAAGGAAAGCCCCTTCAAGGGGATAGGGAAACCGGAGGCGCTAAAGCACCGTAAGCCCC
TCTGGTCACGTCGCATCACAAAAGAACATCGCCTGGTGTACTACGTTGAGCAAGGCGATCTGTACATTGTCGCGTGTCGT
TATCACTACACCACGATCTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9036.05 Da        Isoelectric Point: 4.4180

>AT78761 WP_052932205.1 NZ_CP022273:c4725857-4725612 [Citrobacter freundii]
MQHVNYTDARQHLADLMQQANDDRAPILVTRKGAKSVVMIDAEEFASIQETLHLFSNPANAAHIEASLASLERGDYEELD
I

Download         Length: 246 bp

>AT78761 NZ_CP022273:c4725857-4725612 [Citrobacter freundii]
ATGCAACACGTAAACTATACCGATGCTCGACAGCATTTAGCTGACCTGATGCAGCAGGCTAATGATGATCGTGCGCCAAT
CCTGGTTACTCGTAAGGGTGCTAAAAGCGTGGTAATGATCGACGCGGAAGAGTTCGCATCGATTCAGGAAACGTTACATC
TGTTCAGTAATCCGGCGAACGCCGCACACATTGAAGCCAGCCTTGCATCACTCGAACGCGGCGATTATGAGGAACTGGAC
ATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5H6P158

Antitoxin

Source ID Structure
AlphaFold DB A0A5H6P2W8

References