Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3153855..3154549 Replicon chromosome
Accession NZ_CP022229
Organism Escherichia coli strain WCHEC96200

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag DBR04_RS17265 Protein ID WP_001521903.1
Coordinates 3154151..3154549 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DBR04_RS17260 Protein ID WP_000554758.1
Coordinates 3153855..3154148 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR04_RS17235 3148970..3149719 + 750 WP_016233951.1 C40 family peptidase -
DBR04_RS17240 3149776..3150129 - 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
DBR04_RS17245 3150208..3151947 - 1740 WP_021523009.1 flagellar biosynthesis protein FlhA -
DBR04_RS17250 3151892..3152677 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
DBR04_RS17255 3152748..3153803 + 1056 WP_001226177.1 DNA polymerase IV -
DBR04_RS17260 3153855..3154148 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DBR04_RS17265 3154151..3154549 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DBR04_RS17270 3154559..3155011 + 453 WP_023144376.1 GNAT family N-acetyltransferase -
DBR04_RS17275 3155256..3155462 + 207 Protein_2917 RtcB family protein -
DBR04_RS26880 3155670..3155810 + 141 WP_166739706.1 peptide chain release factor -
DBR04_RS17285 3155867..3157324 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
DBR04_RS17290 3157585..3158043 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
DBR04_RS17295 3158135..3159379 + 1245 WP_000189541.1 esterase FrsA -
- 3158639..3158719 + 81 NuclAT_10 - -
- 3158639..3158719 + 81 NuclAT_10 - -
- 3158639..3158719 + 81 NuclAT_10 - -
- 3158639..3158719 + 81 NuclAT_10 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T78707 WP_001521903.1 NZ_CP022229:3154151-3154549 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T78707 NZ_CP022229:3154151-3154549 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT78707 WP_000554758.1 NZ_CP022229:3153855-3154148 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT78707 NZ_CP022229:3153855-3154148 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References