Detailed information of TA system TA078707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3153855..3154549
• Replicon: chromosome
• Accession: NZ_CP022229
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: DBR04_RS17265
• Protein ID: WP_001521903.1
• Coordinates: 3154151..3154549 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DBR04_RS17260
• Protein ID: WP_000554758.1
• Coordinates: 3153855..3154148 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS17235	3148970..3149719	+	750	WP_016233951.1	C40 family peptidase	-
DBR04_RS17240	3149776..3150129	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
DBR04_RS17245	3150208..3151947	-	1740	WP_021523009.1	flagellar biosynthesis protein FlhA	-
DBR04_RS17250	3151892..3152677	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
DBR04_RS17255	3152748..3153803	+	1056	WP_001226177.1	DNA polymerase IV	-
DBR04_RS17260	3153855..3154148	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DBR04_RS17265	3154151..3154549	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DBR04_RS17270	3154559..3155011	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
DBR04_RS17275	3155256..3155462	+	207	Protein_2917	RtcB family protein	-
DBR04_RS26880	3155670..3155810	+	141	WP_166739706.1	peptide chain release factor	-
DBR04_RS17285	3155867..3157324	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
DBR04_RS17290	3157585..3158043	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DBR04_RS17295	3158135..3159379	+	1245	WP_000189541.1	esterase FrsA	-
-	3158639..3158719	+	81	NuclAT_10	-	-
-	3158639..3158719	+	81	NuclAT_10	-	-
-	3158639..3158719	+	81	NuclAT_10	-	-
-	3158639..3158719	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T78707 WP_001521903.1 NZ_CP022229:3154151-3154549 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T78707 NZ_CP022229:3154151-3154549 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT78707 WP_000554758.1 NZ_CP022229:3153855-3154148 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT78707 NZ_CP022229:3153855-3154148 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3