Detailed information of TA system TA078700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2197269..2197871
• Replicon: chromosome
• Accession: NZ_CP022229
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DBR04_RS12605
• Protein ID: WP_000897305.1
• Coordinates: 2197269..2197580 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBR04_RS12610
• Protein ID: WP_000356395.1
• Coordinates: 2197581..2197871 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS12585	2192310..2192747	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DBR04_RS12590	2192792..2193733	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DBR04_RS12595	2194149..2195963	+	1815	WP_020233236.1	hypothetical protein	-
DBR04_RS12600	2196132..2197040	-	909	WP_001306651.1	alpha/beta hydrolase	-
DBR04_RS12605	2197269..2197580	+	312	WP_000897305.1	hypothetical protein	Toxin
DBR04_RS12610	2197581..2197871	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
DBR04_RS12620	2198230..2198508	+	279	WP_001306650.1	hypothetical protein	-
DBR04_RS12630	2198927..2199145	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DBR04_RS12635	2199364..2199606	+	243	WP_021523315.1	hypothetical protein	-
DBR04_RS12640	2199788..2200717	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
DBR04_RS12645	2200714..2201349	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DBR04_RS12650	2201346..2202248	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T78700 WP_000897305.1 NZ_CP022229:2197269-2197580 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T78700 NZ_CP022229:2197269-2197580 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT78700 WP_000356395.1 NZ_CP022229:2197581-2197871 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT78700 NZ_CP022229:2197581-2197871 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7