Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 48069..48571 Replicon chromosome
Accession NZ_CP022229
Organism Escherichia coli strain WCHEC96200

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DBR04_RS02215 Protein ID WP_000767829.1
Coordinates 48069..48323 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DBR04_RS02220 Protein ID WP_001259255.1
Coordinates 48320..48571 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR04_RS02185 43084..43311 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DBR04_RS02190 43325..44383 - 1059 WP_000492324.1 transport protein YeeE -
DBR04_RS02195 44562..45920 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DBR04_RS02200 45910..45972 - 63 WP_010723108.1 membrane protein YoeI -
DBR04_RS02205 46187..47116 - 930 WP_023154044.1 LysR family transcriptional regulator -
DBR04_RS02210 47162..47986 - 825 WP_000754758.1 SDR family oxidoreductase -
DBR04_RS02215 48069..48323 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DBR04_RS02220 48320..48571 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBR04_RS02225 48854..48904 + 51 WP_001364200.1 his operon leader peptide -
DBR04_RS02230 49050..49949 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
DBR04_RS02235 49955..51259 + 1305 WP_021523144.1 histidinol dehydrogenase -
DBR04_RS02240 51256..52326 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
DBR04_RS02245 52326..53393 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T78693 WP_000767829.1 NZ_CP022229:c48323-48069 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T78693 NZ_CP022229:c48323-48069 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT78693 WP_001259255.1 NZ_CP022229:c48571-48320 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT78693 NZ_CP022229:c48571-48320 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References