Detailed information of TA system TA078692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 75794..76365
• Replicon: plasmid p1_000200
• Accession: NZ_CP022228
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DBR04_RS01735
• Protein ID: WP_032323275.1
• Coordinates: 76084..76365 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBR04_RS01730
• Protein ID: WP_032323276.1
• Coordinates: 75794..76075 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS01695	71480..72271	-	792	WP_063074813.1	hypothetical protein	-
DBR04_RS01700	72434..72736	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBR04_RS01705	72807..73145	+	339	WP_000806445.1	HigA family addiction module antidote protein	-
DBR04_RS01710	73202..73402	+	201	WP_001022420.1	hypothetical protein	-
DBR04_RS01715	73424..74152	+	729	WP_080077649.1	hypothetical protein	-
DBR04_RS01720	74186..75379	-	1194	WP_063074560.1	hypothetical protein	-
DBR04_RS01725	75372..75701	-	330	WP_000542383.1	hypothetical protein	-
DBR04_RS01730	75794..76075	-	282	WP_032323276.1	HigA family addiction module antidote protein	Antitoxin
DBR04_RS01735	76084..76365	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBR04_RS01740	76702..77355	-	654	WP_000410954.1	hypothetical protein	-
DBR04_RS01745	77639..78247	+	609	WP_032323273.1	hypothetical protein	-
DBR04_RS01750	78433..78660	+	228	WP_001396850.1	hypothetical protein	-
DBR04_RS01755	78657..78968	-	312	WP_000717724.1	hypothetical protein	-
DBR04_RS01760	79028..79954	-	927	WP_072275558.1	Rha family transcriptional regulator	-
DBR04_RS01765	79954..80169	-	216	WP_022630900.1	host cell division inhibitor Icd-like protein	-
DBR04_RS01770	80188..80307	-	120	WP_071526433.1	ash family protein	-
DBR04_RS26750	80481..80648	-	168	WP_162983138.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92538	92538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10606.14 Da
• Isoelectric Point: 10.2589

>T78692 WP_032323275.1 NZ_CP022228:c76365-76084 [Escherichia coli]
MIKSFRHKGLERFFKTGSTAGIQAKHAVKLRIQLTALNTAKRPDDMSAPGWELHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T78692 NZ_CP022228:c76365-76084 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACGCTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTGACTGCGCTTAATACAGCTAAACGCCCGGACGATATGTCTGCGCCTGGATGGGAATTGC
ACCCTCTAAAGGGGGCGGACCTTAAAGGTCACTGGGCAATATCGGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGAGAAGACGCAATTCTAGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10160.63 Da
• Isoelectric Point: 7.3179

>AT78692 WP_032323276.1 NZ_CP022228:c76075-75794 [Escherichia coli]
MSRMHNPAHPGAVLREYLGDMSVTDAAAALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TTRPNIRPLFFHA

Nucleotide

• Length: 282 bp

>AT78692 NZ_CP022228:c76075-75794 [Escherichia coli]
ATGAGTCGTATGCATAATCCCGCGCATCCAGGCGCGGTTCTTCGTGAATATTTGGGAGACATGTCAGTCACAGACGCCGC
TGCGGCATTAGGCATTACTCGCACGGCGCTTTCCCGAATTCTGAATGAAAAAGCAGGTATATCTGCTGATATGGCACTTC
GTCTGGAGTCCGCTCTTGGAACCAGTGCCGAAATGTGGACCGGTATGCAATCCCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCACTCGCCCAAACATCCGTCCATTATTTTTCCACGCATAA