Detailed information of TA system TA078691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 72434..73145
• Replicon: plasmid p1_000200
• Accession: NZ_CP022228
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: DBR04_RS01700
• Protein ID: WP_000162415.1
• Coordinates: 72434..72736 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBR04_RS01705
• Protein ID: WP_000806445.1
• Coordinates: 72807..73145 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS01670	67453..68133	-	681	WP_000812238.1	hypothetical protein	-
DBR04_RS01675	68117..69022	-	906	WP_087503502.1	recombination-associated protein RdgC	-
DBR04_RS01680	69082..69735	-	654	WP_087503501.1	baseplate protein	-
DBR04_RS01685	69732..70712	-	981	WP_069936439.1	tail length tape measure protein	-
DBR04_RS01690	70716..71483	-	768	WP_000203293.1	hypothetical protein	-
DBR04_RS01695	71480..72271	-	792	WP_063074813.1	hypothetical protein	-
DBR04_RS01700	72434..72736	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBR04_RS01705	72807..73145	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
DBR04_RS01710	73202..73402	+	201	WP_001022420.1	hypothetical protein	-
DBR04_RS01715	73424..74152	+	729	WP_080077649.1	hypothetical protein	-
DBR04_RS01720	74186..75379	-	1194	WP_063074560.1	hypothetical protein	-
DBR04_RS01725	75372..75701	-	330	WP_000542383.1	hypothetical protein	-
DBR04_RS01730	75794..76075	-	282	WP_032323276.1	HigA family addiction module antidote protein	-
DBR04_RS01735	76084..76365	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBR04_RS01740	76702..77355	-	654	WP_000410954.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92538	92538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T78691 WP_000162415.1 NZ_CP022228:72434-72736 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T78691 NZ_CP022228:72434-72736 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT78691 WP_000806445.1 NZ_CP022228:72807-73145 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT78691 NZ_CP022228:72807-73145 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTTCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3