Detailed information of TA system TA078686

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128158..128391
• Replicon: plasmid pCTXM15_000200
• Accession: NZ_CP022227
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DBR04_RS01105
• Protein ID: WP_001312861.1
• Coordinates: 128158..128316 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	128360..128391 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS01085	123668..124489	-	822	WP_001234426.1	DUF945 domain-containing protein	-
DBR04_RS01090	124607..124894	-	288	WP_000107535.1	hypothetical protein	-
DBR04_RS01100	126096..127763	+	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
DBR04_RS01105	128158..128316	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	128360..128391	-	32	NuclAT_1	-	Antitoxin
-	128360..128391	-	32	NuclAT_1	-	Antitoxin
-	128360..128391	-	32	NuclAT_1	-	Antitoxin
-	128360..128391	-	32	NuclAT_1	-	Antitoxin
-	129833..130030	-	198	NuclAT_0	-	-
-	129833..130030	-	198	NuclAT_0	-	-
-	129833..130030	-	198	NuclAT_0	-	-
-	129833..130030	-	198	NuclAT_0	-	-
DBR04_RS26725	129842..130030	+	189	WP_001299721.1	hypothetical protein	-
DBR04_RS01115	130042..130761	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
DBR04_RS01120	130758..131192	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
DBR04_RS01125	131247..131444	-	198	Protein_156	hypothetical protein	-
DBR04_RS01130	131472..131705	-	234	WP_000005990.1	DUF905 family protein	-
DBR04_RS01135	131773..132312	-	540	WP_000290840.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr	-	1..150853	150853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T78686 WP_001312861.1 NZ_CP022227:c128316-128158 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T78686 NZ_CP022227:c128316-128158 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT78686 NZ_CP022227:c128391-128360 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin