Detailed information of TA system TA078682

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85739..85993
• Replicon: plasmid pCTXM15_000200
• Accession: NZ_CP022227
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DBR04_RS00850
• Protein ID: WP_015387399.1
• Coordinates: 85739..85945 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85932..85993 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS00815	80986..81897	-	912	WP_000440183.1	carbamate kinase	-
DBR04_RS00820	81908..83128	-	1221	WP_000410951.1	arginine deiminase	-
DBR04_RS00825	84021..84503	+	483	Protein_100	VENN motif pre-toxin domain-containing protein	-
DBR04_RS00830	84449..84895	-	447	Protein_101	incFII family plasmid replication initiator RepA	-
DBR04_RS00835	84888..84962	-	75	WP_032336874.1	RepA leader peptide Tap	-
DBR04_RS00845	85198..85455	-	258	WP_000083833.1	replication regulatory protein RepA	-
DBR04_RS00850	85739..85945	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	85932..85993	+	62	NuclAT_2	-	Antitoxin
-	85932..85993	+	62	NuclAT_2	-	Antitoxin
-	85932..85993	+	62	NuclAT_2	-	Antitoxin
-	85932..85993	+	62	NuclAT_2	-	Antitoxin
DBR04_RS00860	86132..86314	-	183	WP_000968309.1	hypothetical protein	-
DBR04_RS00865	86415..87031	+	617	Protein_106	IS1 family transposase	-
DBR04_RS00870	87069..88640	-	1572	WP_023149734.1	IS66 family transposase	-
DBR04_RS00875	88660..89007	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
DBR04_RS00880	89007..89684	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
DBR04_RS26720	89739..89828	+	90	Protein_110	IS1 family transposase	-
DBR04_RS00895	90129..90341	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr	-	1..150853	150853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T78682 WP_015387399.1 NZ_CP022227:c85945-85739 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T78682 NZ_CP022227:c85945-85739 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT78682 NZ_CP022227:85932-85993 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin