Detailed information of TA system TA078624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131675..132096
• Replicon: plasmid pM160133_p2
• Accession: NZ_CP022166
• Organism: Escherichia coli strain M160133

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CE141_RS27025
• Protein ID: WP_001302184.1
• Coordinates: 131938..132096 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	131675..131873 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE141_RS28550	126778..127287	-	510	WP_071600123.1	hypothetical protein	-
CE141_RS27000	127605..128144	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
CE141_RS27005	128201..128434	+	234	WP_000005990.1	DUF905 family protein	-
CE141_RS27010	128500..130458	+	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
CE141_RS27015	130513..130947	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
CE141_RS27020	130944..131663	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	131675..131873	+	199	NuclAT_0	-	Antitoxin
-	131675..131873	+	199	NuclAT_0	-	Antitoxin
-	131675..131873	+	199	NuclAT_0	-	Antitoxin
-	131675..131873	+	199	NuclAT_0	-	Antitoxin
CE141_RS27025	131938..132096	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CE141_RS28555	132334..132693	-	360	Protein_126	hypothetical protein	-
CE141_RS28560	132763..132969	+	207	WP_000547965.1	hypothetical protein	-
CE141_RS27035	132994..133281	+	288	WP_000107546.1	hypothetical protein	-
CE141_RS27040	133400..134221	+	822	WP_001234475.1	DUF945 domain-containing protein	-
CE141_RS27045	134516..135106	-	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
CE141_RS27050	135458..135841	+	384	WP_072647915.1	relaxosome protein TraM	-
CE141_RS27055	136033..136680	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CE141_RS27060	136816..137031	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..173624	173624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T78624 WP_001302184.1 NZ_CP022166:131938-132096 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T78624 NZ_CP022166:131938-132096 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT78624 NZ_CP022166:131675-131873 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin