Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3862602..3863296 Replicon chromosome
Accession NZ_CP022164
Organism Escherichia coli strain M160133

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CE141_RS19620 Protein ID WP_001263491.1
Coordinates 3862602..3863000 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CE141_RS19625 Protein ID WP_000554755.1
Coordinates 3863003..3863296 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE141_RS19590 3857864..3859321 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
CE141_RS19595 3859330..3859611 + 282 WP_022645226.1 hypothetical protein -
CE141_RS19600 3859628..3860137 - 510 WP_001361775.1 hydrolase -
CE141_RS19605 3860199..3860813 - 615 WP_022645225.1 peptide chain release factor H -
CE141_RS19610 3860810..3861949 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
CE141_RS19615 3862140..3862592 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
CE141_RS19620 3862602..3863000 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CE141_RS19625 3863003..3863296 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CE141_RS19630 3863348..3864403 - 1056 WP_022645222.1 DNA polymerase IV -
CE141_RS19635 3864474..3865259 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
CE141_RS19640 3865231..3866943 + 1713 Protein_3634 flagellar biosynthesis protein FlhA -
CE141_RS19645 3867041..3867814 - 774 WP_022645219.1 C40 family peptidase -
CE141_RS19650 3868000..3868260 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T78615 WP_001263491.1 NZ_CP022164:c3863000-3862602 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T78615 NZ_CP022164:c3863000-3862602 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT78615 WP_000554755.1 NZ_CP022164:c3863296-3863003 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT78615 NZ_CP022164:c3863296-3863003 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References