Detailed information of TA system TA078598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 656966..657765
• Replicon: chromosome
• Accession: NZ_CP022164
• Organism: Escherichia coli strain M160133

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: CE141_RS03400
• Protein ID: WP_000347273.1
• Coordinates: 656966..657430 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: CE141_RS03405
• Protein ID: WP_001308975.1
• Coordinates: 657430..657765 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE141_RS03365	651967..652401	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
CE141_RS03370	652419..653297	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CE141_RS03375	653287..654066	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CE141_RS03380	654077..654550	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CE141_RS03385	654573..655853	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CE141_RS03395	656102..656911	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CE141_RS03400	656966..657430	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CE141_RS03405	657430..657765	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CE141_RS03410	657914..659485	-	1572	WP_077516436.1	galactarate dehydratase	-
CE141_RS03415	659860..661194	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
CE141_RS03420	661210..661980	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T78598 WP_000347273.1 NZ_CP022164:c657430-656966 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T78598 NZ_CP022164:c657430-656966 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT78598 WP_001308975.1 NZ_CP022164:c657765-657430 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT78598 NZ_CP022164:c657765-657430 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08