Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 61806..62533 | Replicon | plasmid pABWA45_1 |
| Accession | NZ_CP022155 | ||
| Organism | Escherichia coli strain ABWA45 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A663AUB1 |
| Locus tag | CES94_RS27180 | Protein ID | WP_000558568.1 |
| Coordinates | 61806..62117 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | CES94_RS27185 | Protein ID | WP_000990392.1 |
| Coordinates | 62114..62533 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CES94_RS27150 | 57128..57825 | + | 698 | Protein_70 | IS1 family transposase | - |
| CES94_RS27155 | 57835..58917 | + | 1083 | WP_032140505.1 | lytic murein transglycosylase B | - |
| CES94_RS27160 | 59025..59882 | - | 858 | Protein_72 | APC family permease | - |
| CES94_RS27165 | 59935..60639 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| CES94_RS27170 | 60704..61165 | + | 462 | Protein_74 | lactate utilization protein C | - |
| CES94_RS27175 | 61215..61595 | - | 381 | Protein_75 | gluconate permease | - |
| CES94_RS27180 | 61806..62117 | + | 312 | WP_000558568.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| CES94_RS27185 | 62114..62533 | + | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| CES94_RS27195 | 63866..64177 | - | 312 | WP_001324897.1 | hypothetical protein | - |
| CES94_RS27200 | 64339..64689 | + | 351 | WP_000928911.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| CES94_RS27205 | 64994..65470 | - | 477 | WP_000043177.1 | DNA starvation/stationary phase protection protein | - |
| CES94_RS27210 | 65594..66541 | - | 948 | Protein_82 | transposase | - |
| CES94_RS27220 | 66607..67311 | + | 705 | WP_088765929.1 | IS6 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..145220 | 145220 | |
| - | inside | IScluster/Tn | - | - | 57322..90856 | 33534 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12372.11 Da Isoelectric Point: 10.0935
>T78593 WP_000558568.1 NZ_CP022155:61806-62117 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
>T78593 NZ_CP022155:61806-62117 [Escherichia coli]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT78593 WP_000990392.1 NZ_CP022155:62114-62533 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
>AT78593 NZ_CP022155:62114-62533 [Escherichia coli]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTTCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTTCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A663AUB1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |