Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3185471..3185973 Replicon chromosome
Accession NZ_CP022154
Organism Escherichia coli strain ABWA45

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CES94_RS17055 Protein ID WP_000767829.1
Coordinates 3185471..3185725 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CES94_RS17060 Protein ID WP_001259255.1
Coordinates 3185722..3185973 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CES94_RS17030 3180486..3180713 - 228 WP_088765796.1 sulfurtransferase TusA family protein -
CES94_RS17035 3180727..3181785 - 1059 WP_000492340.1 transport protein YeeE -
CES94_RS17040 3181964..3183322 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CES94_RS29370 3183312..3183374 - 63 WP_010723108.1 membrane protein YoeI -
CES94_RS17045 3183589..3184518 - 930 WP_061093170.1 LysR family transcriptional regulator -
CES94_RS17050 3184564..3185388 - 825 WP_000754737.1 SDR family oxidoreductase -
CES94_RS17055 3185471..3185725 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CES94_RS17060 3185722..3185973 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CES94_RS17065 3186256..3186306 + 51 WP_001364200.1 his operon leader peptide -
CES94_RS17075 3186452..3187351 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CES94_RS17080 3187357..3188661 + 1305 WP_001572810.1 histidinol dehydrogenase -
CES94_RS17085 3188658..3189728 + 1071 WP_063112832.1 histidinol-phosphate transaminase -
CES94_RS17090 3189728..3190795 + 1068 WP_032248774.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T78585 WP_000767829.1 NZ_CP022154:c3185725-3185471 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T78585 NZ_CP022154:c3185725-3185471 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT78585 WP_001259255.1 NZ_CP022154:c3185973-3185722 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT78585 NZ_CP022154:c3185973-3185722 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
ACTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References