Detailed information of TA system TA078571

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2304614..2304985
• Replicon: chromosome
• Accession: NZ_CP022154
• Organism: Escherichia coli strain ABWA45

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: CES94_RS12070
• Protein ID: WP_001317028.1
• Coordinates: 2304791..2304985 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2304614..2304792 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES94_RS12045	2300569..2301942	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
CES94_RS12050	2302071..2303006	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CES94_RS12055	2303058..2304293	-	1236	WP_000040852.1	site-specific integrase	-
CES94_RS12060	2304295..2304510	-	216	WP_000079604.1	excisionase XisR	-
CES94_RS12065	2304589..2304798	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2304614..2304792	+	179	NuclAT_0	-	Antitoxin
-	2304614..2304792	+	179	NuclAT_0	-	Antitoxin
-	2304614..2304792	+	179	NuclAT_0	-	Antitoxin
-	2304614..2304792	+	179	NuclAT_0	-	Antitoxin
CES94_RS12070	2304791..2304985	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CES94_RS12075	2305042..2305851	-	810	WP_000166319.1	recombination protein RecT	-
CES94_RS12080	2305844..2308444	-	2601	WP_001549183.1	exodeoxyribonuclease VIII	-
CES94_RS12085	2308546..2308821	-	276	WP_000632297.1	protein RacC	-
CES94_RS12090	2308896..2309066	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CES94_RS12095	2309066..2309287	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2296092..2314309	18217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T78571 WP_001317028.1 NZ_CP022154:c2304985-2304791 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T78571 NZ_CP022154:c2304985-2304791 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT78571 NZ_CP022154:2304614-2304792 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin