Detailed information of TA system TA078566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1180719..1181446
• Replicon: chromosome
• Accession: NZ_CP022154
• Organism: Escherichia coli strain ABWA45

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: CES94_RS06185
• Protein ID: WP_000547564.1
• Coordinates: 1180719..1181030 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CES94_RS06190
• Protein ID: WP_000126294.1
• Coordinates: 1181027..1181446 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES94_RS06155	1175861..1177570	+	1710	WP_001288140.1	formate hydrogenlyase subunit HycE	-
CES94_RS06160	1177580..1178122	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
CES94_RS06165	1178122..1178889	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
CES94_RS06170	1178886..1179296	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CES94_RS06175	1179289..1179759	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CES94_RS06180	1179784..1180545	+	762	WP_001026446.1	hypothetical protein	-
CES94_RS06185	1180719..1181030	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
CES94_RS06190	1181027..1181446	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CES94_RS06195	1181560..1182984	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
CES94_RS06200	1182993..1184450	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CES94_RS06205	1184710..1185720	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
CES94_RS06210	1185869..1186396	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T78566 WP_000547564.1 NZ_CP022154:1180719-1181030 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T78566 NZ_CP022154:1180719-1181030 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT78566 WP_000126294.1 NZ_CP022154:1181027-1181446 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT78566 NZ_CP022154:1181027-1181446 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5