Detailed information of TA system TA078562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 766156..766849
• Replicon: chromosome
• Accession: NZ_CP022154
• Organism: Escherichia coli strain ABWA45

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: CES94_RS04030
• Protein ID: WP_000415583.1
• Coordinates: 766156..766452 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: CES94_RS04035
• Protein ID: WP_000650107.1
• Coordinates: 766454..766849 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES94_RS29120	761290..762482	+	1193	Protein_723	Hcp family type VI secretion system effector	-
CES94_RS04005	762489..762821	+	333	WP_000914691.1	DUF2645 family protein	-
CES94_RS04010	762867..764216	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
CES94_RS04015	764213..764872	-	660	WP_001221493.1	two-component system response regulator QseB	-
CES94_RS04020	765024..765416	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
CES94_RS04025	765469..765951	+	483	WP_000609258.1	GyrI-like domain-containing protein	-
CES94_RS04030	766156..766452	+	297	WP_000415583.1	type II toxin-antitoxin system toxin MqsR	Toxin
CES94_RS04035	766454..766849	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
CES94_RS04040	766982..768589	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
CES94_RS04045	768727..770985	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	761772..790848	29076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11216.99 Da
• Isoelectric Point: 8.9070

>T78562 WP_000415583.1 NZ_CP022154:766156-766452 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGLV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T78562 NZ_CP022154:766156-766452 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCTGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT78562 WP_000650107.1 NZ_CP022154:766454-766849 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT78562 NZ_CP022154:766454-766849 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LU65