Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2646640..2647225 Replicon chromosome
Accession NZ_CP022129
Organism Methylovulum psychrotolerans strain HV10_M2

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CEK71_RS12020 Protein ID WP_088619614.1
Coordinates 2646640..2646954 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CEK71_RS12025 Protein ID WP_088619615.1
Coordinates 2646944..2647225 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEK71_RS11990 2643064..2643462 + 399 WP_088619609.1 hypothetical protein -
CEK71_RS23190 2643632..2644486 + 855 WP_198328193.1 HNH endonuclease -
CEK71_RS12000 2644511..2645068 + 558 WP_088619610.1 hypothetical protein -
CEK71_RS12005 2645167..2645415 + 249 WP_088619611.1 BrnT family toxin -
CEK71_RS12010 2645412..2645654 + 243 WP_088619612.1 BrnA antitoxin family protein -
CEK71_RS12015 2645800..2646324 + 525 WP_088619613.1 hypothetical protein -
CEK71_RS12020 2646640..2646954 + 315 WP_088619614.1 transcriptional regulator Toxin
CEK71_RS12025 2646944..2647225 + 282 WP_088619615.1 helix-turn-helix domain-containing protein Antitoxin
CEK71_RS12030 2647409..2650951 - 3543 WP_088619616.1 exodeoxyribonuclease V subunit beta -
CEK71_RS12035 2651167..2651832 + 666 WP_088619617.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(7-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-81)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11614.31 Da        Isoelectric Point: 9.3927

>T78523 WP_088619614.1 NZ_CP022129:2646640-2646954 [Methylovulum psychrotolerans]
MYTVAETSIFRKYASEIWSDAEVTEFINWIAANPYSGAVISGSGGCRKVRWSRSGKGKRGGARVIYFVIENDAIWLLIAY
TKAKFDNLPTAFLAELKKEVENGL

Download         Length: 315 bp

>T78523 NZ_CP022129:2646640-2646954 [Methylovulum psychrotolerans]
ATGTACACAGTCGCCGAAACGTCTATTTTCCGCAAATACGCATCAGAAATATGGTCAGATGCCGAAGTGACGGAATTCAT
CAACTGGATTGCTGCAAACCCTTATTCGGGTGCGGTCATTTCTGGTTCTGGTGGTTGCAGGAAAGTCCGTTGGTCGCGTT
CGGGCAAAGGTAAGCGTGGCGGAGCGCGGGTCATTTACTTTGTTATTGAAAATGATGCCATTTGGCTGTTGATTGCTTAC
ACTAAAGCAAAATTCGACAATCTGCCAACGGCGTTTTTAGCAGAACTGAAAAAAGAGGTGGAAAATGGACTCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10292.74 Da        Isoelectric Point: 8.8279

>AT78523 WP_088619615.1 NZ_CP022129:2646944-2647225 [Methylovulum psychrotolerans]
MDSEMERFQQDLLESVKQMKRGEAVRVSNVALTEAAQARSFVGLSQTEFAKLLGVSARTLQDWEQGRREPTGAAKTLLKV
AVSHPEVLRGLHA

Download         Length: 282 bp

>AT78523 NZ_CP022129:2646944-2647225 [Methylovulum psychrotolerans]
ATGGACTCTGAAATGGAACGGTTTCAGCAAGATTTGCTTGAGTCAGTCAAACAAATGAAACGTGGCGAAGCTGTCAGGGT
ATCTAACGTAGCCCTTACTGAGGCCGCACAGGCCCGGTCGTTTGTTGGCCTTTCCCAAACTGAGTTTGCAAAATTGCTTG
GCGTGTCAGCACGAACTTTACAAGATTGGGAACAAGGCAGGCGTGAACCCACCGGGGCCGCTAAAACCTTGCTGAAAGTG
GCAGTAAGCCACCCAGAAGTGCTTAGGGGGCTTCATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References