Detailed information of TA system TA078502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 96862..97519
• Replicon: plasmid p1605752AC2
• Accession: NZ_CP022126
• Organism: Klebsiella pneumoniae strain DHQP1605752_NV

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: CEV20_RS01700
• Protein ID: WP_000270043.1
• Coordinates: 96862..97212 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CEV20_RS01705
• Protein ID: WP_000124640.1
• Coordinates: 97217..97519 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEV20_RS01660	93336..93833	-	498	WP_000062185.1	hypothetical protein	-
CEV20_RS01665	93836..94324	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
CEV20_RS01670	94421..94756	+	336	WP_000683477.1	hypothetical protein	-
CEV20_RS01675	94771..95241	-	471	WP_001281821.1	hypothetical protein	-
CEV20_RS01680	95234..95605	-	372	WP_000516918.1	hypothetical protein	-
CEV20_RS01685	95616..95810	-	195	WP_000343597.1	hypothetical protein	-
CEV20_RS01695	96151..96699	-	549	WP_001061573.1	hypothetical protein	-
CEV20_RS01700	96862..97212	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEV20_RS01705	97217..97519	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
CEV20_RS01710	97546..97839	-	294	WP_001239998.1	hypothetical protein	-
CEV20_RS01715	97928..98200	-	273	WP_001043046.1	HU family DNA-binding protein	-
CEV20_RS01720	98258..98785	-	528	WP_004201083.1	thermonuclease family protein	-
CEV20_RS29735	98788..99093	-	306	WP_171820680.1	hypothetical protein	-
CEV20_RS01730	99005..99862	-	858	WP_001167036.1	hypothetical protein	-
CEV20_RS01735	99849..100079	-	231	WP_000972665.1	hypothetical protein	-
CEV20_RS01740	100079..100597	-	519	WP_000210757.1	nitrite reductase	-
CEV20_RS01745	100594..101040	-	447	WP_000919343.1	hypothetical protein	-
CEV20_RS01750	101040..101399	-	360	WP_000422769.1	hypothetical protein	-
CEV20_RS01755	101457..101885	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1 / rmtC / sul1 / qacE / aac(6')-Ib / blaCMY-6	htpB	1..140133	140133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T78502 WP_000270043.1 NZ_CP022126:96862-97212 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T78502 NZ_CP022126:96862-97212 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT78502 WP_000124640.1 NZ_CP022126:97217-97519 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT78502 NZ_CP022126:97217-97519 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9