Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1303014..1303616 Replicon chromosome
Accession NZ_CP022111
Organism Nitrospirillum amazonense CBAmc

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag Y958_RS18980 Protein ID WP_088873506.1
Coordinates 1303014..1303295 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag Y958_RS18985 Protein ID WP_088874090.1
Coordinates 1303332..1303616 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y958_RS18950 1298491..1299504 + 1014 WP_088873500.1 NADPH:quinone oxidoreductase family protein -
Y958_RS18955 1299570..1300187 + 618 WP_088873501.1 TetR/AcrR family transcriptional regulator -
Y958_RS18960 1300299..1300979 + 681 WP_088873502.1 type 1 glutamine amidotransferase domain-containing protein -
Y958_RS18965 1301027..1302127 + 1101 WP_088873503.1 NADH:flavin oxidoreductase/NADH oxidase -
Y958_RS18970 1302140..1302439 + 300 WP_088873504.1 antibiotic biosynthesis monooxygenase -
Y958_RS18975 1302564..1302941 + 378 WP_088873505.1 ACT domain-containing protein -
Y958_RS18980 1303014..1303295 + 282 WP_088873506.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Y958_RS18985 1303332..1303616 + 285 WP_088874090.1 HigA family addiction module antidote protein Antitoxin
Y958_RS18990 1303679..1304629 - 951 WP_088873507.1 FAD-binding protein -
Y958_RS18995 1304632..1305381 - 750 WP_088873508.1 electron transfer flavoprotein subunit beta/FixA family protein -
Y958_RS19000 1305394..1306521 - 1128 WP_088873509.1 acyl-CoA dehydrogenase -
Y958_RS19005 1306668..1307690 + 1023 WP_186291345.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(19-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-64)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10434.85 Da        Isoelectric Point: 8.0935

>T78473 WP_088873506.1 NZ_CP022111:1303014-1303295 [Nitrospirillum amazonense CBAmc]
MIKSTKGKISEAVLTGTLTKGFPADLVRAAQRKLAILHAATTVDDLRVPPGNRLEKLSGDREGQHSIRINDQWRVCFRWD
GQDAHDVEIVDYH

Download         Length: 282 bp

>T78473 NZ_CP022111:1303014-1303295 [Nitrospirillum amazonense CBAmc]
ATGATTAAAAGCACCAAGGGCAAGATCAGTGAGGCGGTGCTCACGGGCACGTTGACGAAAGGTTTTCCTGCGGACTTGGT
GCGCGCGGCGCAGCGGAAATTGGCGATCCTTCACGCGGCGACCACCGTGGACGATCTGCGCGTTCCGCCCGGCAATCGCT
TGGAAAAGCTGTCGGGAGACCGTGAAGGCCAGCATTCCATCCGCATCAACGATCAATGGCGCGTGTGTTTCCGCTGGGAC
GGCCAAGATGCCCATGATGTTGAAATCGTCGATTATCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10295.81 Da        Isoelectric Point: 7.4248

>AT78473 WP_088874090.1 NZ_CP022111:1303332-1303616 [Nitrospirillum amazonense CBAmc]
MRPVHPGEVLREEFMEPLGLTAGKIAKACGVPRTRIERVASEQTEMTADTALRLAKALGTTAQFWLNLQTTHNLARAHQS
VDLSAVQVLHHAAE

Download         Length: 285 bp

>AT78473 NZ_CP022111:1303332-1303616 [Nitrospirillum amazonense CBAmc]
ATGCGCCCCGTCCATCCCGGCGAAGTCCTACGGGAGGAGTTCATGGAGCCCCTGGGCCTGACAGCGGGCAAGATCGCCAA
GGCCTGCGGCGTGCCCCGCACACGGATCGAACGGGTGGCGAGCGAACAGACGGAAATGACGGCCGATACCGCCCTGCGCC
TGGCCAAGGCGCTGGGCACCACGGCGCAGTTCTGGCTGAACCTGCAGACCACGCACAACCTGGCCCGCGCCCATCAGAGC
GTAGACCTGTCGGCCGTGCAGGTGTTGCACCACGCTGCGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References