Detailed information of TA system TA078440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 279767..280467
• Replicon: chromosome
• Accession: NZ_CP022102
• Organism: Vibrio anguillarum strain JLL237

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: CEJ46_RS15510
• Protein ID: WP_064626987.1
• Coordinates: 280075..280467 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A191W9Z3
• Locus tag: CEJ46_RS15505
• Protein ID: WP_064626657.1
• Coordinates: 279767..280060 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEJ46_RS15485	275155..276024	+	870	WP_064626653.1	DMT family transporter	-
CEJ46_RS15490	276227..277324	-	1098	WP_064626654.1	calcium:proton antiporter	-
CEJ46_RS15495	277555..278364	-	810	WP_064626655.1	TerC family protein	-
CEJ46_RS15500	278630..279352	-	723	WP_064626656.1	chromosome partitioning protein ParB	-
CEJ46_RS15505	279767..280060	+	294	WP_064626657.1	hypothetical protein	Antitoxin
CEJ46_RS15510	280075..280467	+	393	WP_064626987.1	type II toxin-antitoxin system YafO family toxin	Toxin
CEJ46_RS15515	280834..281322	-	489	WP_064626658.1	GNAT family N-acetyltransferase	-
CEJ46_RS15520	281523..282422	+	900	WP_064626659.1	LysR family transcriptional regulator	-
CEJ46_RS15525	282689..283453	-	765	WP_045591510.1	hypothetical protein	-
CEJ46_RS15530	284082..284489	+	408	WP_000864702.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15199.28 Da
• Isoelectric Point: 6.9109

>T78440 WP_064626987.1 NZ_CP022102:280075-280467 [Vibrio anguillarum]
IRIFKHKILIDSLTESELQSLTKDFRHYKETGQKPDFFGRDEAYDHPNTLPILKSEEVRHIHLAASDAPFLSSIQFYQTS
DKHLVYCQGWNDLSCFLLIAILTPDAHEQARNRTIMHNLGLIAEKFRQQF

Nucleotide

• Length: 393 bp

>T78440 NZ_CP022102:280075-280467 [Vibrio anguillarum]
ATTAGAATTTTTAAGCATAAGATCTTAATTGATTCACTTACTGAAAGCGAGCTTCAATCTTTGACTAAAGACTTTAGACA
CTATAAGGAGACTGGTCAAAAACCAGATTTTTTTGGTCGAGATGAAGCTTATGATCATCCCAATACATTACCGATCTTGA
AGTCAGAGGAAGTTAGGCACATTCACTTAGCCGCTAGTGATGCTCCGTTCTTAAGTTCTATCCAATTTTACCAAACGAGT
GATAAGCACTTAGTGTATTGTCAAGGCTGGAATGACCTTAGTTGTTTTCTGTTAATCGCCATCCTTACACCTGACGCTCA
TGAGCAAGCCCGTAATCGAACTATCATGCATAACTTGGGTTTGATAGCTGAAAAATTTAGGCAGCAATTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10574.99 Da
• Isoelectric Point: 4.8172

>AT78440 WP_064626657.1 NZ_CP022102:279767-280060 [Vibrio anguillarum]
MKTSTIYAEKAVSSSEARKHFKDCLSGEPVVVLSNNEPAGYMMSASFFEGLMKALEGKNADSVSSFRPMSARLEVIAHLN
AQALTELNESILGEFQE

Nucleotide

• Length: 294 bp

>AT78440 NZ_CP022102:279767-280060 [Vibrio anguillarum]
ATGAAAACAAGTACAATCTATGCAGAAAAAGCAGTGTCATCAAGCGAAGCTAGAAAGCATTTTAAAGATTGCTTATCAGG
GGAGCCAGTCGTAGTTTTATCGAACAATGAGCCTGCTGGTTATATGATGAGCGCTTCTTTTTTTGAAGGACTGATGAAAG
CTTTAGAAGGCAAGAATGCTGACTCTGTTTCATCATTTAGGCCAATGAGTGCTCGCTTAGAAGTCATTGCTCATCTGAAT
GCTCAAGCATTAACAGAGCTTAATGAGAGTATTTTGGGTGAGTTTCAAGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A191W9Z3